direct primary antibodies cd11a antibody  (R&D Systems)

Journal: BMC Cancer

Article Title: LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro

doi: 10.1186/1471-2407-12-455

Figure Lengend Snippet: Primer sequences and PCR settings

Article Snippet: Specific direct primary antibodies CD11a antibody (FAB35951A) and CD18 (FAB1730P) from R&D system (Minneapolis, MN, USA) or isotypic control antibody (BD Pharmingen, San Diego, CA, USA) were used at 1 μg ml.

Techniques: Hybridization

Journal: BMC Cancer

Article Title: LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro

doi: 10.1186/1471-2407-12-455

Figure Lengend Snippet: Expression of CD11a and CD18 in melanoma A375, 1205LU and SLM8 cell lines with conditioned medium. Cell-surface expression of CD11a and CD18 on indicated melanoma cell lines treated for 24 hrs with HUVEC conditioned medium was analyzed by flow cytometry. Isotypic controls are represented as empty histograms and specific antibody-labelling is displayed as shaded histograms. Histograms obtained with cells incubated with FCS-complete medium and labeled with specific antibodies, which overlap with the isotypic control are not shown. Data from obtained with 3 independent experiments.

Article Snippet: Specific direct primary antibodies CD11a antibody (FAB35951A) and CD18 (FAB1730P) from R&D system (Minneapolis, MN, USA) or isotypic control antibody (BD Pharmingen, San Diego, CA, USA) were used at 1 μg ml.

Techniques: Expressing, Flow Cytometry, Incubation, Labeling, Control

Journal: BMC Cancer

Article Title: LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro

doi: 10.1186/1471-2407-12-455

Figure Lengend Snippet: Effect of CD11a and CD18-blocking antibodies on the transendothelial migration of A375, 1205LU and SLM8 cell lines. The experiments were performed as detailed in Figure , except that 2μg/ml of CD11a or CD18-blocking antibodies were introduced in the upper chamber of the Transwells when indicated. Histograms represent 3 independent experiments. In each experiment each condition was analyzed in duplicate.

Article Snippet: Specific direct primary antibodies CD11a antibody (FAB35951A) and CD18 (FAB1730P) from R&D system (Minneapolis, MN, USA) or isotypic control antibody (BD Pharmingen, San Diego, CA, USA) were used at 1 μg ml.

Techniques: Blocking Assay, Migration

Journal: BMC Cancer

Article Title: LFA-1 and ICAM-1 expression induced during melanoma-endothelial cell co-culture favors the transendothelial migration of melanoma cell lines in vitro

doi: 10.1186/1471-2407-12-455

Figure Lengend Snippet: Effect of CD11 and CD18-blocking antibodies on the formation of clumps. A Semi-quantitative PCRs were performed to detect the expression of the ICAM-1 transcript. GAPDH is used as a DNA amount control. B A375, 1205LU and SLM8 cell lines were treated with 2 μg/ml of CD11a or CD18-blocking antibodies as indicated. Melanoma cells were labeled with DiO then fixed and labeled with DAPI prior to their observation under an epifluorescence microscope using a magnification of x10. Data were obtained from 3 independent experiments.

Article Snippet: Specific direct primary antibodies CD11a antibody (FAB35951A) and CD18 (FAB1730P) from R&D system (Minneapolis, MN, USA) or isotypic control antibody (BD Pharmingen, San Diego, CA, USA) were used at 1 μg ml.

Techniques: Blocking Assay, Expressing, Control, Labeling, Microscopy

Journal: The Canadian Journal of Cardiology

Article Title: Effect of interleukin-15 on the course of myocarditis in Coxsackievirus B3-infected BALB/c mice

doi:

Figure Lengend Snippet: Immunohistochemical evaluation

Article Snippet: According to the staining method described by Kühl et al ( 20 ), specific monoclonal rat antimouse primary antibodies directed against the leukocyte surface antigens CD4, CD8a, CD11a and CD11b (Becton Dickinson PharMingen, USA), and CD3 and CD54/ICAM-1 (Dianova, Germany) were embedded for 60 min. Unbound antibodies were eluted twice with phosphate-buffered saline (PBS) for 5 min each.

Techniques: Immunohistochemical staining, Control

Journal: The Canadian Journal of Cardiology

Article Title: Effect of interleukin-15 on the course of myocarditis in Coxsackievirus B3-infected BALB/c mice

doi:

Figure Lengend Snippet: CD11b staining reflecting infiltration of macrophages in the myocardium. A In the myocardium of a normal, sham-infected mouse, no significant infiltrating cells are found. B Infiltrating CD11b-positive cells (arrows) in the heart of a Coxsackivirus B3 (CVB3)-infected mouse. C After treatment with interleukin (IL)-15, cellular infiltrates are significantly reduced. The white arrow marks a single CD11b-positive cell. D Treatment with IL-15 fusion protein did not decrease the number of cellular infiltrates compared with CVB3-infected animals. The arrows indicate a cluster of CD11b cellular infiltrates

Article Snippet: According to the staining method described by Kühl et al ( 20 ), specific monoclonal rat antimouse primary antibodies directed against the leukocyte surface antigens CD4, CD8a, CD11a and CD11b (Becton Dickinson PharMingen, USA), and CD3 and CD54/ICAM-1 (Dianova, Germany) were embedded for 60 min. Unbound antibodies were eluted twice with phosphate-buffered saline (PBS) for 5 min each.

Techniques: Staining, Infection